Description
Description
MW= 8.6 kDa calculated. Full length Ubiquitin cloned from human cDNA, expressed in E. coli. The protein consists of the human ubiquitin (residues 1-76, UniProtKB accession P0CG47).
Sequence 10 20 30 40 50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70
EDGRTLSDYN IQKESTLHLV LRLRGG
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
Supplied as
1 mg/mL solution in phosphate buffer 50 mM pH 7, NaN3 0.02%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1490 M-1cm-1 calculated).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2mM.
Storage
-20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.