Sequence
30 40 50
M-KIEE GKLVIWINGD KGYNGLAEVG
60 70 80 90 100
KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG
110 120 130 140 150
LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN
160 170 180 190 200
PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG
210 220 230 240 250
KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM
260 270 280 290 300
TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE
310 320 330 340 350
LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA
360 370 380 390
QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QT
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating between 35.0 and 45.0 kDa.
Supplied as
1.0 mg/mL solution in phosphate buffer 20 mM pH 7.2, EDTA 0.1 mM, NaN3 3 mM, Maltotriose 3 mM, Pefabloc® (AEBSF) 50 uM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 66350 M-1cm-1 calculated).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 1.5 mM.
Storage
-20°C. The protein is stable at 4°C for months.
Avoid repeated freeze/thaw cycles.